The sanctuary area spreads from Kalasubai to Harischandragad in Akole Tehsil of Ahmadnagar district. The area is part of Sahyadri hill ranges. The Kalsubai sanctuary is challenge to trekkers as it is most rugged, hilly area and difficult to get accessibility. Kalasubai is the highest (1646 m) peak of Western Ghats in the Maharashtra State.
The vegetation type is southern moist mixed deciduous. The part of Sahyadri that is close to West Coast is semi evergreen forest and semi deciduous shrub savanna. The main tree species that are seen in this area are Hirda, Jambhul, Chandawa, Bahava, Kumbhal, Gulchavi, Kudal, Siras, Kharvel, Karap, Avali, Aashind, Lokhandi, Beheda and acharacter worth noticing.
Species that are undergrowth are Mandar, Kadipatta, Ghaneri, Chilhar, Parjambhual, Karvi, Karwand, Ber, Dhaiti, and Rametha. There are large patches of grasslands mostly of Marvel, Dongari, Pawanya, Ber, Surad, Harali etc. You can also see Bamboo in this region but rarely.
Tourists can find wide variety of mammals, reptiles and birds. The mammals found here are Leopard, Jungle cat, Palm civet, Mongoose, Hyena, Wolf, Jackal, Fox, Wild Boar, Barking Deer, Sambar, Hare, and Bats etc. The most attractive animals are Indian Giant Squirrel and Porcupine.
The reptiles found in this sanctuary are Monitor Lizard, Fan-Throated Lizard, Turtles and many species of snakes. Among the birds are the common hill and grass land birds. One can also spot water birds such as White Necked Storks, Black Ibis, Herons, Egrets, Cormorants, Water hen's, etc